Hotspot

rpoC2

LSC · 15964-20121 · 4158 bp

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Pi 0.00159

MarkerSeek score 40.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 15
pi: 0.00159
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGGAGATTTTGCCAAGTCAA	GATCTGTTGCAGGAATGGGA	40.0	50.0	55.1	57.6	243 bp	1.0	54.4
rpoC2_p2	AGGAGATTTTGCCAAGTCAA	AGATCTGTTGCAGGAATGGG	40.0	50.0	55.1	57.6	244 bp	1.0	54.3
rpoC2_p3	AGGAGATTTTGCCAAGTCAA	CCCAGATCTGTTGCAGGAAT	40.0	50.0	55.1	57.6	247 bp	1.0	54.3
rpoC2_p4	AGGAGATTTTGCCAAGTCAA	TTTAGCAACGCCTTTCTCCC	40.0	50.0	55.1	58.5	265 bp	1.0	54.0
rpoC2_p5	AGGAGATTTTGCCAAGTCAA	TTTCTCCCCAGATCTGTTGC	40.0	50.0	55.1	57.5	253 bp	1.0	53.8