Hotspot

cemA

LSC · 59282-59971 · 690 bp

Back to Linaria

Pi 0.00348

MarkerSeek score 53.70

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 5
pi: 0.00348
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	CCGACGAATGAAGTGGGTTA	CCCCCGGATAGAAAGTTTTT	50.0	45.0	57.6	55.3	821 bp	1.0	55.9
cemA_p2	CACATAGAGCCGACGAATGA	CCCCCGGATAGAAAGTTTTT	50.0	45.0	57.5	55.3	830 bp	1.0	55.0
cemA_p3	TCACATAGAGCCGACGAATG	CCCCCGGATAGAAAGTTTTT	50.0	45.0	57.5	55.3	831 bp	1.0	55.0
cemA_p4	TAGAGCCGACGAATGAAGTG	CCCCCGGATAGAAAGTTTTT	50.0	45.0	57.4	55.3	826 bp	1.0	54.4
cemA_p5	GATCACATAGAGCCGACGAA	CCCCCGGATAGAAAGTTTTT	50.0	45.0	57.2	55.3	833 bp	1.0	52.7