Hotspot

ndhG-ndhI

SSC · 115546-115884 · 339 bp

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Pi 0.0183

MarkerSeek score 54.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 339
variable_sites: 15
pi: 0.0183
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAATCGTGTATTGGTCCCGG	TCACCTCAAACAAGAAACGT	50.0	40.0	58.0	55.4	392-400 bp	1.0	62.6
ndhG-ndhI_p2	ATCGTGTATTGGTCCCGGTA	TCACCTCAAACAAGAAACGT	50.0	40.0	58.2	55.4	390-398 bp	1.0	61.1
ndhG-ndhI_p3	AAATCGTGTATTGGTCCCGG	TTCACCTCAAACAAGAAACGT	50.0	38.1	58.0	56.2	393-401 bp	1.0	60.9
ndhG-ndhI_p4	CGTGTATTGGTCCCGGTAAA	TCACCTCAAACAAGAAACGT	50.0	40.0	57.6	55.4	388-396 bp	1.0	59.8
ndhG-ndhI_p5	ATCGTGTATTGGTCCCGGTA	TTCACCTCAAACAAGAAACGT	50.0	38.1	58.2	56.2	391-399 bp	1.0	59.4