Hotspot

rpl32-trnL-UAG

SSC · 110367-111018 · 652 bp

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Pi 0.0093

MarkerSeek score 47.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 652
variable_sites: 11
pi: 0.0093
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGGAAAGGTTGTTGGGCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	784-802 bp	1.0	82.0
rpl32-trnL-UAG_p2	AAAGGAAAGGTTGTTGGGCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	785-803 bp	1.0	82.0
rpl32-trnL-UAG_p3	CGCACAGACGTTTTTGAACA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	920-938 bp	1.0	81.3
rpl32-trnL-UAG_p4	CGCACAGACGTTTTTGAACA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	921-939 bp	1.0	81.3
rpl32-trnL-UAG_p5	ACGCACAGACGTTTTTGAAC	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	921-939 bp	1.0	81.3