Hotspot

psbE-petL

LSC · 62871-63707 · 837 bp

Back to Linaria

Pi 0.00581

MarkerSeek score 43.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 10
pi: 0.00581
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCGAAATAACTAGTTATAGTAAGCA	50.0	32.0	57.8	54.6	884-891 bp	1.0	41.6
psbE-petL_p2	ACTGACGAATAACCAACCCG	CCGAAATAACTAGTTATAGTAAGCA	50.0	32.0	57.9	54.6	974-981 bp	1.0	41.5
psbE-petL_p3	ACCCGCAATAAACAGGGAAG	CCGAAATAACTAGTTATAGTAAGCA	50.0	32.0	58.2	54.6	959-966 bp	1.0	41.5
psbE-petL_p4	CCGCAATAAACAGGGAAGGT	CCGAAATAACTAGTTATAGTAAGCA	50.0	32.0	58.2	54.6	957-964 bp	1.0	41.5
psbE-petL_p5	ACGAATAACCAACCCGCAAT	CCGAAATAACTAGTTATAGTAAGCA	45.0	32.0	58.2	54.6	970-977 bp	1.0	41.5