Hotspot

psbF-psbE

LSC · 62605-62618 · 14 bp

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Pi 0.0429
MarkerSeek score 62.00
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
14
variable_sites
1
pi
0.0429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 GAACAGCCAACCATCGTACT CTGGCCGCTTTGATTCTTTG 50.0 50.0 57.9 58.0 119 bp 1.0 79.8
psbF-psbE_p2 GAACAGCCAACCATCGTACT GAGCCGACAAGGAATTCCAT 50.0 50.0 57.9 58.0 145 bp 1.0 79.4
psbF-psbE_p3 AACAGCCAACCATCGTACTG CTGGCCGCTTTGATTCTTTG 50.0 50.0 58.2 58.0 118 bp 1.0 79.0
psbF-psbE_p4 GTGAACAGCCAACCATCGTA CTGGCCGCTTTGATTCTTTG 50.0 50.0 58.2 58.0 121 bp 1.0 79.0
psbF-psbE_p5 TGAACAGCCAACCATCGTAC CTGGCCGCTTTGATTCTTTG 50.0 50.0 58.2 58.0 120 bp 1.0 79.0