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psbZ-trnG-GCC

LSC · 34750-34994 · 245 bp

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Pi 0.00574

MarkerSeek score 52.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 245
variable_sites: 3
pi: 0.00574
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	404-413 bp	1.0	80.6
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	405-414 bp	1.0	80.6
psbZ-trnG-GCC_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	402-411 bp	1.0	80.2
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	403-412 bp	1.0	80.2
psbZ-trnG-GCC_p5	CCGTCGTATTTGCTTCTCCT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	413-422 bp	1.0	79.9