Hotspot

trnS-GCU-trnG-UCC

LSC · 7889-8555 · 667 bp

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Pi 0.0051

MarkerSeek score 52.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 7
pi: 0.0051
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AGGAATAAAACCGAAAGACCCT	50.0	40.9	58.0	57.3	780-782 bp	1.0	59.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AGGAATAAAACCGAAAGACCCT	50.0	40.9	58.0	57.3	779-781 bp	1.0	59.6
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AGGAATAAAACCGAAAGACCCT	50.0	40.9	58.2	57.3	834-836 bp	1.0	58.3
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AGGAATAAAACCGAAAGACCCTT	50.0	39.1	58.0	58.0	780-782 bp	1.0	56.7
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	AGGAATAAAACCGAAAGACCCT	50.0	40.9	58.4	57.3	782-784 bp	1.0	56.7