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trnK-UUU-rps16

LSC · 4119-4811 · 693 bp

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Pi 0.00837

MarkerSeek score 59.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 12
pi: 0.00837
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	815-829 bp	1.0	66.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	814-828 bp	1.0	66.9
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	GTGCTCAACCCACAGGAAC	50.0	57.9	57.8	58.7	900-914 bp	1.0	66.2
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	GTGCTCAACCCACAGGAAC	50.0	57.9	57.8	58.7	899-913 bp	1.0	66.1
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	GTGCTCAACCCACAGGAAC	50.0	57.9	58.3	58.7	829-843 bp	1.0	66.1