Hotspot

ndhA

SSC · 122562-124562 · 2001 bp

Back to Limonium

Pi 0.012

MarkerSeek score 44.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2001
variable_sites: 58
pi: 0.012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTACCTTGCCACTCGTACTG	GGGTTTGTCAGGACCAATGT	55.0	50.0	58.0	58.0	2700-2827 bp	1.0	82.0
ndhA_p2	GTACCTTGCCACTCGTACTG	AATTGGGGTTTGTCAGGACC	55.0	50.0	58.0	58.0	2705-2832 bp	1.0	82.0
ndhA_p3	ACCTTGCCCAATGTACCTTG	GGGTTTGTCAGGACCAATGT	50.0	50.0	58.1	58.0	2712-2839 bp	1.0	81.5
ndhA_p4	ACCTTGCCCAATGTACCTTG	AATTGGGGTTTGTCAGGACC	50.0	50.0	58.1	58.0	2717-2844 bp	1.0	81.4
ndhA_p5	GTACCTTGCCACTCGTACTG	TCCGAATTGGTGAAATGGCA	55.0	45.0	58.0	58.1	2563-2690 bp	1.0	81.4