Hotspot

ndhH-rps15

SSC · 125746-125833 · 88 bp

Back to Limonium

Pi 0.0352

MarkerSeek score 59.40

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 7
pi: 0.0352
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TGCATTGATGGGTGATGAGG	TGGATAAATAGCTGCGCACA	50.0	45.0	57.9	57.7	630-634 bp	1.0	79.6
ndhH-rps15_p2	ACATTGGTCCTGACAAACCC	TGGATAAATAGCTGCGCACA	50.0	45.0	58.0	57.7	1232-1236 bp	1.0	79.2
ndhH-rps15_p3	GGTCCTGACAAACCCCAATT	TGGATAAATAGCTGCGCACA	50.0	45.0	58.0	57.7	1227-1231 bp	1.0	79.2
ndhH-rps15_p4	ACATTGGTCCTGACAAACCC	GGTGGATAAATAGCTGCGCA	50.0	50.0	58.0	58.4	1238 bp	0.2	44.7
ndhH-rps15_p5	GGTCCTGACAAACCCCAATT	GGTGGATAAATAGCTGCGCA	50.0	50.0	58.0	58.4	1233 bp	0.2	44.7