Hotspot

trnL-UAG-ccsA

SSC · 117252-117384 · 133 bp

Back to Limonium

Pi 0.0346

MarkerSeek score 72.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 133
variable_sites: 10
pi: 0.0346
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	ATTTGAAACAAGCCGCCATG	GCAATTACAATTGAAACGGTCG	45.0	40.9	57.9	57.2	272-292 bp	1.0	63.8
trnL-UAG-ccsA_p2	TAGACACGCTGCTCTTAGGA	GCAATTACAATTGAAACGGTCG	50.0	40.9	57.9	57.2	242-262 bp	1.0	63.8
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	GCAATTACAATTGAAACGGTCG	45.0	40.9	57.8	57.2	249-269 bp	1.0	63.2
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	GCAATTACAATTGAAACGGTCG	55.0	40.9	57.8	57.2	244-264 bp	1.0	63.2
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	GCAATTACAATTGAAACGGTCG	55.0	40.9	57.8	57.2	243-263 bp	1.0	63.2