Hotspot

rpl32-trnL-UAG

SSC · 116365-117171 · 807 bp

Back to Limonium

Pi 0.0295

MarkerSeek score 71.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 807
variable_sites: 57
pi: 0.0295
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATCTCTCTCGACCGGAAAGT	AAGCACTGCTTCCTAAGAGC	50.0	50.0	57.9	57.9	890-908 bp	1.0	86.6
rpl32-trnL-UAG_p2	ATCTCTCTCGACCGGAAAGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	880-898 bp	1.0	86.6
rpl32-trnL-UAG_p3	ATCTCTCTCGACCGGAAAGT	GGATTGCAAAGCGGGTACTA	50.0	50.0	57.9	58.0	1436-1471 bp	1.0	86.0
rpl32-trnL-UAG_p4	ATCTCTCTCGACCGGAAAGT	AGGCCTGAAGTAGCAAACAC	50.0	50.0	57.9	58.1	1387-1422 bp	1.0	85.6
rpl32-trnL-UAG_p5	ATCTCTCTCGACCGGAAAGT	GGCCTGAAGTAGCAAACACT	50.0	50.0	57.9	58.1	1386-1421 bp	1.0	85.6