Hotspot

psaJ-rpl33

LSC · 67226-67740 · 515 bp

Back to Limonium

Pi 0.0221

MarkerSeek score 64.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 515
variable_sites: 28
pi: 0.0221
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGCGCTTTAGCAGGTCTATT	ATAGCGAGAAATGCCCCTTG	50.0	50.0	58.0	58.0	686-698 bp	1.0	86.1
psaJ-rpl33_p2	CGGTAGGAAAGATGCAGCAT	ATAGCGAGAAATGCCCCTTG	50.0	50.0	58.0	58.0	979-980 bp	1.0	84.8
psaJ-rpl33_p3	AAAACAAACGCGCTACCAAG	ATAGCGAGAAATGCCCCTTG	45.0	50.0	57.9	58.0	1226-1229 bp	1.0	84.2
psaJ-rpl33_p4	TTCGGTAGGAAAGATGCAGC	ATAGCGAGAAATGCCCCTTG	50.0	50.0	58.0	58.0	981 bp	0.8	71.9
psaJ-rpl33_p5	TTCGGTAGGAAAGATGCAGC	TAATTGCAACCGACTGGGAG	50.0	50.0	58.0	57.9	1026 bp	0.8	71.3