Hotspot

psbM-trnD-GUC

LSC · 29708-30584 · 877 bp

Back to Limonium

Pi 0.0115

MarkerSeek score 62.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 24
pi: 0.0115
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCATTTTGGCTGACCGTTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.4	58.5	1036-1045 bp	1.0	66.9
psbM-trnD-GUC_p2	AATCATTTTGGCTGACCGTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.2	58.5	1038-1047 bp	1.0	65.0
psbM-trnD-GUC_p3	ATCATTTTGGCTGACCGTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.2	58.5	1037-1046 bp	1.0	65.0
psbM-trnD-GUC_p4	TCATTTTGGCTGACCGTTTTT	TTCAATTGGTCAGAGCACCG	38.1	50.0	57.2	58.5	1036-1045 bp	1.0	64.8
psbM-trnD-GUC_p5	TTTGGCTGACCGTTTTTACA	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.1	58.5	1032-1041 bp	1.0	64.1