Hotspot

atpH-atpI

LSC · 13164-13919 · 756 bp

Back to Limonium

Pi 0.013

MarkerSeek score 60.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 23
pi: 0.013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACAGAAGCGGCCGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	805-843 bp	1.0	84.9
atpH-atpI_p2	ATAACAGAAGCGGCCGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	775-813 bp	1.0	84.6
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	861-899 bp	1.0	84.1
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	821-859 bp	1.0	83.9
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	831-869 bp	1.0	83.9