Hotspot

ndhE-psaC

SSC · 119268-119546 · 279 bp

Back to Limnophila

Pi 0.0541

MarkerSeek score 80.30

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 279
variable_sites: 14
pi: 0.0541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	GCTATTGGCCTGGCTATTGT	TACATCCGTCCCAGGGTATC	50.0	55.0	58.0	58.0	451-466 bp	1.0	85.8
ndhE-psaC_p2	GCTATTGGCCTGGCTATTGT	TCCGTCCCAGGGTATCATTT	50.0	50.0	58.0	58.1	447-462 bp	1.0	85.0
ndhE-psaC_p3	GCTATTGGCCTGGCTATTGT	ATCCGTCCCAGGGTATCATT	50.0	50.0	58.0	57.9	448-463 bp	1.0	84.9
ndhE-psaC_p4	GCTATTGGCCTGGCTATTGT	TTCTAAAACATCGGTGGGGC	50.0	50.0	58.0	58.2	429-444 bp	1.0	84.8
ndhE-psaC_p5	GCTATTGGCCTGGCTATTGT	TTTACATCCGTCCCAGGGTA	50.0	50.0	58.0	57.7	453-468 bp	1.0	83.8