Hotspot

psbT-pbf1

LSC · 73613-73678 · 66 bp

Back to Limnophila

Pi 0.0606

MarkerSeek score 71.90

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 4
pi: 0.0606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	TCGAGAACCGCCTAAAGTTC	ATCCATTCGAAGAACACGGG	50.0	50.0	57.6	58.0	126-127 bp	1.0	79.5
psbT-pbf1_p2	GAGAACCGCCTAAAGTTCCA	ATCCATTCGAAGAACACGGG	50.0	50.0	57.5	58.0	124-125 bp	1.0	78.6
psbT-pbf1_p3	CGAGAACCGCCTAAAGTTCC	ATCCATTCGAAGAACACGGG	55.0	50.0	58.6	58.0	125-126 bp	1.0	77.2
psbT-pbf1_p4	AACCGCCTAAAGTTCCAACT	ATCCATTCGAAGAACACGGG	45.0	50.0	57.3	58.0	121-122 bp	1.0	77.2
psbT-pbf1_p5	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	50.0	55.0	57.6	57.7	175-176 bp	1.0	76.7