Hotspot

psaJ-rpl33

LSC · 66684-67032 · 349 bp

Back to Limnophila

Pi 0.0867

MarkerSeek score 78.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 30
pi: 0.0867
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	ACTCGCGCATCTTTCTTCTT	45.0	45.0	58.0	57.9	416-708 bp	1.0	83.8
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	ACTCGCGCATCTTTCTTCTT	45.0	45.0	58.1	57.9	407-699 bp	1.0	83.5
psaJ-rpl33_p3	TTTTCCCAGATGCGTTGACA	CAAAATTACCGTGACTCGCG	45.0	50.0	58.0	57.9	429-721 bp	1.0	83.3
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	CAAAATTACCGTGACTCGCG	45.0	50.0	58.1	57.9	420-712 bp	1.0	82.9
psaJ-rpl33_p5	TTCCCAGATGCGTTGACATT	ACTCGCGCATCTTTCTTCTT	45.0	45.0	57.8	57.9	414-706 bp	1.0	82.4