Hotspot

trnL-UAA-trnF-GAA

LSC · 47786-48061 · 276 bp

Back to Limnophila

Pi 0.0449

MarkerSeek score 76.40

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 276
variable_sites: 12
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TCTACCAGCTGAGCTATCCC	45.0	55.0	57.8	58.0	380-389 bp	1.0	82.8
trnL-UAA-trnF-GAA_p2	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	416-425 bp	1.0	82.0
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	TCAGTCCTCTGCTCTACCAG	45.0	55.0	57.8	57.9	392-401 bp	1.0	81.6
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	321-330 bp	1.0	81.3
trnL-UAA-trnF-GAA_p5	ACATGTCAATACCGGCAACA	AACCATGTGTCAGGAACCAG	45.0	50.0	57.8	57.7	437-446 bp	1.0	80.1