Hotspot

atpA-atpF

LSC · 11457-11513 · 57 bp

Back to Limnophila

Pi 0.0755

MarkerSeek score 76.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 4
pi: 0.0755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATTGCTCGATACGTTCACGG	TATGTTGGGGGCGATGAAAG	50.0	50.0	58.4	57.9	145-149 bp	1.0	80.4
atpA-atpF_p2	ATTGCTCGATACGTTCACGG	TTGGGGGCGATGAAAGAAAT	50.0	45.0	58.4	57.8	141-145 bp	1.0	79.1
atpA-atpF_p3	ATTGCTCGATACGTTCACGG	GTATGTTGGGGGCGATGAAA	50.0	50.0	58.4	58.2	146-150 bp	1.0	78.8
atpA-atpF_p4	GCTCGATACGTTCACGGATA	TATGTTGGGGGCGATGAAAG	50.0	50.0	57.3	57.9	142-146 bp	1.0	78.6
atpA-atpF_p5	ATTGCTCGATACGTTCACGG	ATATTGGTATGTTGGGGGCG	50.0	50.0	58.4	57.7	152-156 bp	1.0	78.3