Hotspot

trnS-GCU-trnG-UCC

LSC · 7841-8814 · 974 bp

Back to Limnophila

Pi 0.0681
MarkerSeek score 80.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
974
variable_sites
62
pi
0.0681
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 981-1029 bp 1.0 56.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 980-1028 bp 1.0 56.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 982-1030 bp 1.0 55.7
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 981-1029 bp 1.0 55.7
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA TACCACTAAACTATACCCGC 50.0 45.0 58.2 54.0 1035-1083 bp 1.0 55.4