Hotspot

ycf1

SSC · 121729-127305 · 5577 bp

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Pi 0.00805
MarkerSeek score 53.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5577
variable_sites
424
pi
0.00805
unique_haplotype_count
62
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GGGTCGGTTGTAATTACATCG CGATTCACGCGCAAATATAAA 47.6 38.1 56.8 55.8 234-240 bp 0.878788 43.2
ycf1_p2 GGGTCGGTTGTAATTACATCGA CGATTCACGCGCAAATATAAA 45.5 38.1 58.2 55.8 234-240 bp 0.878788 43.2
ycf1_p3 GGGTCGGTTGTAATTACATCG CGATTCACGCGCAAATATAA 47.6 40.0 56.8 55.0 234-240 bp 0.818182 40.7
ycf1_p4 GGGTCGGTTGTAATTACATCGA CGATTCACGCGCAAATATAA 45.5 40.0 58.2 55.0 234-240 bp 0.818182 40.7
ycf1_p5 TGGGTCGGTTGTAATTACATCG CGATTCACGCGCAAATATAA 45.5 40.0 58.5 55.0 235-241 bp 0.818182 40.7