Hotspot

trnR-ACG-rrn5S

IRa · 128269-128518 · 250 bp

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Pi 0.00684
MarkerSeek score 51.70
Primer available yes
Label trnR-ACG-rrn5S

Feature Metadata

label_name
trnR-ACG-rrn5S
feature_type
igs
region
IRa
length_bp
250
variable_sites
13
pi
0.00684
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5S_p1 CCACGTGCTCTAATCCTCTG GTTAAACTCTACTGCGGCGA 55.0 50.0 57.8 58.0 255-356 bp 1.0 85.6
trnR-ACG-rrn5S_p2 CCACGTGCTCTAATCCTCTG TTAAACTCTACTGCGGCGAC 55.0 50.0 57.8 58.0 254-355 bp 1.0 85.6
trnR-ACG-rrn5S_p3 CCACGTGCTCTAATCCTCTG TGGTTAAACTCTACTGCGGC 55.0 50.0 57.8 57.9 257-358 bp 1.0 85.0
trnR-ACG-rrn5S_p4 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 275-376 bp 1.0 84.5
trnR-ACG-rrn5S_p5 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 276-377 bp 1.0 84.5