Hotspot

rps15-ycf1

SSC · 121325-121728 · 404 bp

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Pi 0.0286
MarkerSeek score 65.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
404
variable_sites
69
pi
0.0286
unique_haplotype_count
52
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TTCCCAATTGTTCTGGTGCA ACGTCGGAGAGAGTTGAGAA 45.0 50.0 57.9 58.1 1296-1358 bp 1.0 90.5
rps15-ycf1_p2 GGTGATGAGGGCCCATATTG ACGTCGGAGAGAGTTGAGAA 55.0 50.0 58.1 58.1 1083-1145 bp 1.0 90.5
rps15-ycf1_p3 CCCAAGCCATAACAAATGCG ACGTCGGAGAGAGTTGAGAA 50.0 50.0 58.1 58.1 1382-1444 bp 1.0 90.4
rps15-ycf1_p4 TTCCCAATTGTTCTGGTGCA TCCATTGCACAACATAGCGA 45.0 45.0 57.9 57.9 1385-1446 bp 0.939394 87.7
rps15-ycf1_p5 CCCAAGCCATAACAAATGCG TCCATTGCACAACATAGCGA 50.0 45.0 58.1 57.9 1471-1532 bp 0.939394 87.4