Hotspot

ccsA-ndhD

SSC · 113143-113316 · 174 bp

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Pi 0.033
MarkerSeek score 61.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
174
variable_sites
23
pi
0.033
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GCACTTGCGGCTTCTATAGG TCAGTTGACAAGGTACAAGCT 55.0 42.9 58.8 57.2 327-332 bp 1.0 67.6
ccsA-ndhD_p2 GGCACTTGCGGCTTCTATAG TCAGTTGACAAGGTACAAGCT 55.0 42.9 58.8 57.2 328-333 bp 1.0 67.6
ccsA-ndhD_p3 CACTTGCGGCTTCTATAGGA TCAGTTGACAAGGTACAAGCT 50.0 42.9 57.1 57.2 326-331 bp 1.0 66.7
ccsA-ndhD_p4 GCACTTGCGGCTTCTATAGG ATCAGTTGACAAGGTACAAGCT 55.0 40.9 58.8 57.6 328-333 bp 1.0 63.4
ccsA-ndhD_p5 GGCACTTGCGGCTTCTATAG ATCAGTTGACAAGGTACAAGCT 55.0 40.9 58.8 57.6 329-334 bp 1.0 63.4