Hotspot

rrn5-trnR-ACG

IRb · 106051-106300 · 250 bp

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Pi 0.00684
MarkerSeek score 51.70
Primer available yes
Label rrn5-trnR-ACG

Feature Metadata

label_name
rrn5-trnR-ACG
feature_type
igs
region
IRb
length_bp
250
variable_sites
13
pi
0.00684
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-trnR-ACG_p1 GTTAAACTCTACTGCGGCGA CCACGTGCTCTAATCCTCTG 50.0 55.0 58.0 57.8 255-356 bp 1.0 85.6
rrn5-trnR-ACG_p2 TTAAACTCTACTGCGGCGAC CCACGTGCTCTAATCCTCTG 50.0 55.0 58.0 57.8 254-355 bp 1.0 85.6
rrn5-trnR-ACG_p3 TGGTTAAACTCTACTGCGGC CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 257-358 bp 1.0 85.0
rrn5-trnR-ACG_p4 AATCCATCCCGAACTTGGTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 275-376 bp 1.0 84.5
rrn5-trnR-ACG_p5 CAATCCATCCCGAACTTGGT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 276-377 bp 1.0 84.5