Hotspot

trnP-UGG-psaJ

LSC · 64334-64673 · 340 bp

Back to Lilium

Pi 0.015
MarkerSeek score 58.90
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
340
variable_sites
31
pi
0.015
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC TAGACCCGCTAAAGACCCAA 50.0 50.0 58.1 58.1 458-473 bp 1.0 87.3
trnP-UGG-psaJ_p2 ACCCGTGACATTTTGTACCC GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 491-506 bp 1.0 86.8
trnP-UGG-psaJ_p3 CCCGTGACATTTTGTACCCA GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 490-505 bp 1.0 86.8
trnP-UGG-psaJ_p4 ACAGGATTTGAACCCGTGAC GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 502-517 bp 1.0 86.6
trnP-UGG-psaJ_p5 CAGGATTTGAACCCGTGACA GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 501-516 bp 1.0 86.6