Hotspot

psbC-trnS-UGA

LSC · 33691-33827 · 137 bp

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Pi 0.0358
MarkerSeek score 63.60
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
137
variable_sites
17
pi
0.0358
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TTCCATGACCCCTCTTAGCT GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.7 58.1 227-236 bp 1.0 86.9
psbC-trnS-UGA_p2 TTCCATGACCCCTCTTAGCT GATGGCCGAGTGGTTGATAG 50.0 55.0 57.7 58.1 238-247 bp 1.0 85.9
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.2 58.2 181-190 bp 0.924242 84.9
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.2 58.1 235-244 bp 0.924242 84.1
psbC-trnS-UGA_p5 CCTGTTCTTTCCATGACCCC GATGGCCGAGTGGTTGATAG 55.0 55.0 58.2 58.1 246-255 bp 0.924242 83.1