Hotspot

rps2-rpoC2

LSC · 15629-15860 · 232 bp

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Pi 0.0234
MarkerSeek score 61.60
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
232
variable_sites
48
pi
0.0234
unique_haplotype_count
47
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 ACTCCCGCTTCCATCATTTC TGGGACATCACAACAACCAT 50.0 45.0 58.0 57.3 312-340 bp 0.984848 85.7
rps2-rpoC2_p2 ACTCCCGCTTCCATCATTTC CACAACAACCATTTACGCGA 50.0 45.0 58.0 57.3 304-332 bp 0.954545 84.5
rps2-rpoC2_p3 ACTCCCGCTTCCATCATTTC TCACAACAACCATTTACGCG 50.0 45.0 58.0 57.3 305-333 bp 0.954545 84.5
rps2-rpoC2_p4 TTTGCAGAGATGTAAGGGGC CACAACAACCATTTACGCGA 50.0 45.0 57.9 57.3 364-392 bp 0.954545 83.8
rps2-rpoC2_p5 TTTGCAGAGATGTAAGGGGC TCACAACAACCATTTACGCG 50.0 45.0 57.9 57.3 365-393 bp 0.954545 83.8