Hotspot

atpH-atpI

LSC · 13023-13907 · 885 bp

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Pi 0.00996
MarkerSeek score 63.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
885
variable_sites
82
pi
0.00996
unique_haplotype_count
55
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 629-995 bp 1.0 86.5
atpH-atpI_p2 AATAACGGAAGCGGCAGAAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.2 58.5 630-996 bp 1.0 85.3
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.8 58.5 644-1010 bp 1.0 84.9
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT TAGGTGAATCCATGGAGGGT 45.0 50.0 58.0 57.4 602-968 bp 0.969697 84.2
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA TAGGTGAATCCATGGAGGGT 45.0 50.0 58.2 57.4 603-969 bp 0.969697 83.0