Hotspot

trnK-UUU-rps16

LSC · 4062-4810 · 749 bp

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Pi 0.0181
MarkerSeek score 72.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
749
variable_sites
120
pi
0.0181
unique_haplotype_count
56
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCTCAACCCACAGTAACTGT 50.0 50.0 58.2 57.7 516-882 bp 1.0 88.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCTCAACCCACAGTAACTGT 50.0 50.0 58.2 57.7 515-881 bp 1.0 88.6
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CTTGAAAATGGCGCTCAACC 50.0 50.0 58.2 58.3 528-894 bp 1.0 88.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAATGGCGCTCAAC 50.0 50.0 58.2 58.3 528-894 bp 1.0 88.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAATGGCGCTCAACC 50.0 50.0 58.2 58.3 527-893 bp 1.0 88.3