Hotspot

clpP1

LSC · 75959-78069 · 2111 bp

Back to Ligustrum

Pi 0.0432

MarkerSeek score 74.20

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2111
variable_sites: 259
pi: 0.0432
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TCTAAACGAGTCGCGCATAC	GCAATGGGGGTTGATACCAT	50.0	50.0	58.2	57.9	2580-2787 bp	1.0	89.6
clpP1_p2	CGTTCTTGACAGAGTGGGAG	GCAATGGGGGTTGATACCAT	55.0	50.0	57.9	57.9	2867-2990 bp	0.923077	85.0
clpP1_p3	CTTGACAGAGTGGGAGCTTC	GCAATGGGGGTTGATACCAT	55.0	50.0	57.9	57.9	2863-2986 bp	0.923077	84.9
clpP1_p4	GTTCTTGACAGAGTGGGAGC	GCAATGGGGGTTGATACCAT	55.0	50.0	58.2	57.9	2866-2989 bp	0.923077	84.0
clpP1_p5	TAGCTGACCCTCTTAGTCCG	GCAATGGGGGTTGATACCAT	55.0	50.0	57.9	57.9	2885-3000 bp	0.846154	82.0