Hotspot

accD

LSC · 62116-64299 · 2184 bp

Back to Ligustrum

Pi 0.0955

MarkerSeek score 72.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2184
variable_sites: 497
pi: 0.0955
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCGATGACGATGACGAGTAC	AATGCGACCCAACAGGAATT	55.0	45.0	58.0	58.1	2914 bp	0.076923	42.3
accD_p2	CCGATGACGATGACGAGTAC	ATGCGACCCAACAGGAATTT	55.0	45.0	58.0	58.1	2913 bp	0.076923	42.3
accD_p3	CCGATGACGATGACGAGTAC	TTAGAATGCGACCCAACAGG	55.0	50.0	58.0	57.9	2918 bp	0.076923	42.1
accD_p4	TCCGATGACGATGACGAGTA	AATGCGACCCAACAGGAATT	50.0	45.0	58.1	58.1	2915 bp	0.076923	42.0
accD_p5	TCCGATGACGATGACGAGTA	ATGCGACCCAACAGGAATTT	50.0	45.0	58.1	58.1	2914 bp	0.076923	42.0