Hotspot

rpl22-rps19

LSC · 89663-89726 · 64 bp

Back to Ligustrum

Pi 0.052

MarkerSeek score 68.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 16
pi: 0.052
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCTTTGTCAGCCGATAGAGA	AGCGATACTAGATCTCGGCG	50.0	55.0	57.4	58.9	154-572 bp	0.846154	69.8
rpl22-rps19_p2	GCTTTGTCAGCCGATAGAGA	GCGATACTAGATCTCGGCGT	50.0	55.0	57.4	59.2	153-571 bp	0.846154	67.5
rpl22-rps19_p3	GCTTTGTCAGCCGATAGAGA	CGATACTAGATCTCGGCGTT	50.0	50.0	57.4	56.7	152-570 bp	0.846154	67.1
rpl22-rps19_p4	GCTTTGTCAGCCGATAGAGA	AAGCGATACTAGATCTCGGC	50.0	50.0	57.4	56.7	155-573 bp	0.846154	66.8
rpl22-rps19_p5	GCTTTGTCAGCCGATAGAGAT	AGCGATACTAGATCTCGGCG	47.6	55.0	57.9	58.9	154-572 bp	0.846154	65.6