Hotspot

psaI-pafII

LSC · 65091-65542 · 452 bp

Back to Ligustrum

Pi 0.0133

MarkerSeek score 57.60

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 19
pi: 0.0133
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	ATTTCCGGCAATTGCAATGG	AAAAAGGATAAGGGCCCAGC	45.0	50.0	58.0	58.1	588-601 bp	1.0	82.9
psaI-pafII_p2	ATTTCCGGCAATTGCAATGG	GGGCCCAGCAGAAATTACTT	45.0	50.0	58.0	58.1	577-590 bp	1.0	82.9
psaI-pafII_p3	ATTTCCGGCAATTGCAATGG	AAGGGCCCAGCAGAAATTAC	45.0	50.0	58.0	58.1	579-592 bp	1.0	82.8
psaI-pafII_p4	TAGGCCTAGTATTTCCGGCA	AAAAAGGATAAGGGCCCAGC	50.0	50.0	57.9	58.1	598-611 bp	1.0	82.3
psaI-pafII_p5	GGCAATTGCAATGGCTTCTT	AAAAAGGATAAGGGCCCAGC	45.0	50.0	57.9	58.1	582-595 bp	1.0	82.3