Hotspot

trnT-GGU-accD

LSC · 59603-62115 · 2513 bp

Back to Ligustrum

Pi 0.145

MarkerSeek score 62.40

Primer available yes

Label trnT-GGU-accD

Feature Metadata

label_name: trnT-GGU-accD
feature_type: igs
region: LSC
length_bp: 2513
variable_sites: 616
pi: 0.145
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.32
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-accD_p1	GAACCGATGACTTACGCCTT	CTTGCAATGACCGCTCCTAT	50.0	50.0	58.0	58.0	2465-2687 bp	0.230769	54.0
trnT-GGU-accD_p2	GAACCGATGACTTACGCCTT	CTGACGATCACTCTCAAGCC	50.0	55.0	58.0	58.1	2496-2718 bp	0.230769	53.6
trnT-GGU-accD_p3	CCATGGCGTTACTCTACCAC	CTTGCAATGACCGCTCCTAT	55.0	50.0	58.1	58.0	2444-2666 bp	0.230769	53.5
trnT-GGU-accD_p4	TGGCGTTACTCTACCACTGA	CTTGCAATGACCGCTCCTAT	50.0	50.0	58.1	58.0	2441-2663 bp	0.230769	53.3
trnT-GGU-accD_p5	GAACCGATGACTTACGCCTT	AGCCCCTCACGTATACTTGT	50.0	50.0	58.0	58.1	2369-2591 bp	0.230769	53.2