Hotspot

rbcL-trnT-GGU

LSC · 58588-59530 · 943 bp

Back to Ligustrum

Pi 0.096

MarkerSeek score 66.20

Primer available yes

Label rbcL-trnT-GGU

Feature Metadata

label_name: rbcL-trnT-GGU
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 182
pi: 0.096
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-trnT-GGU_p1	ACTACAGTTCGGTGGAGGAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1239 bp	0.230769	48.7
rbcL-trnT-GGU_p2	GGCTTCAGGGGGTATTCATG	GTGGTAGAGTAACGCCATGG	55.0	55.0	58.0	58.1	1305 bp	0.230769	48.6
rbcL-trnT-GGU_p3	TTGAATGCTACTGCGGGTAC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1718 bp	0.230769	48.6
rbcL-trnT-GGU_p4	ACTACAGTTCGGTGGAGGAA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1242 bp	0.230769	48.5
rbcL-trnT-GGU_p5	GGCTTCAGGGGGTATTCATG	TCAGTGGTAGAGTAACGCCA	55.0	50.0	58.0	58.1	1308 bp	0.230769	48.4