Hotspot

rps2-rpoC2

LSC · 17363-17561 · 199 bp

Back to Ligustrum

Pi 0.0254

MarkerSeek score 63.60

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 15
pi: 0.0254
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCCAAAATGAACTCCTGCCT	TCCATGATACATCAGAACAATCA	50.0	34.8	58.7	55.5	304-321 bp	1.0	47.4
rps2-rpoC2_p2	CCATGGCCAAAATGAACTCC	TCCATGATACATCAGAACAATCA	50.0	34.8	57.3	55.5	309-326 bp	1.0	47.3
rps2-rpoC2_p3	TTGCCGAGATATAAGGTGCC	TCCATGATACATCAGAACAATCA	50.0	34.8	57.5	55.5	353-370 bp	1.0	46.7
rps2-rpoC2_p4	TGCCGAGATATAAGGTGCCA	TCCATGATACATCAGAACAATCA	50.0	34.8	58.6	55.5	352-369 bp	1.0	46.7
rps2-rpoC2_p5	GCCGAGATATAAGGTGCCAT	TCCATGATACATCAGAACAATCA	50.0	34.8	57.2	55.5	351-368 bp	1.0	46.7