Hotspot

psbI-trnS-GCU

LSC · 9173-9302 · 130 bp

Back to Ligustrum

Pi 0.0108

MarkerSeek score 57.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 5
pi: 0.0108
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	221-227 bp	0.923077	74.1
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	222-228 bp	0.923077	74.0
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	240-246 bp	0.923077	72.9
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.8	58.0	221-227 bp	0.923077	71.7
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	47.6	50.0	57.8	58.0	222-228 bp	0.923077	71.7