Hotspot

ycf1

SSC · 126505-131982 · 5478 bp

Back to Ligusticum

Pi 0.0148

MarkerSeek score 54.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 331
pi: 0.0148
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGGGAGTTCCATGATACTCA	GCTGAGGACTTCGATGAGTG	47.6	55.0	58.3	58.1	1163-1184 bp	1.0	77.5
ycf1_p2	ACGGGAGTTCCATGATACTCA	GAAACCTTGGCACAGATCCA	47.6	50.0	58.3	58.1	1026-1047 bp	1.0	77.5
ycf1_p3	ACGGGAGTTCCATGATACTCA	CCCTTCCCTTTGGTTCTGTC	47.6	55.0	58.3	58.1	919-937 bp	0.833333	70.5
ycf1_p4	ACGGGAGTTCCATGATACTCA	CCTTCCCTTTGGTTCTGTCC	47.6	55.0	58.3	58.1	918-936 bp	0.833333	70.5
ycf1_p5	ACGGGAGTTCCATGATACTCA	CTTCCCTTTGGTTCTGTCCC	47.6	55.0	58.3	58.1	917-935 bp	0.833333	70.5