Hotspot

ndhG-ndhI

SSC · 121474-121834 · 361 bp

Back to Ligusticum

Pi 0.026

MarkerSeek score 79.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 39
pi: 0.026
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	AGGGCGTTTACCAATGTCAA	50.0	45.0	58.0	57.7	490-526 bp	1.0	88.3
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	TGCTTTAGGGCGTTTACCAA	50.0	45.0	58.0	57.7	496-532 bp	1.0	88.2
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	TTGCTTTAGGGCGTTTACCA	50.0	45.0	58.0	57.7	497-533 bp	1.0	88.2
ndhG-ndhI_p4	AATACCACTCCCAGACCTCC	TGCTTTAGGGCGTTTACCAA	55.0	45.0	58.1	57.7	528-558 bp	0.916667	79.8
ndhG-ndhI_p5	AATACCACTCCCAGACCTCC	TTGCTTTAGGGCGTTTACCA	55.0	45.0	58.1	57.7	529-559 bp	0.916667	79.8