Hotspot

trnE-UUC-trnT-GGU

LSC · 32347-33127 · 781 bp

Back to Ligusticum

Pi 0.0386
MarkerSeek score 71.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
781
variable_sites
103
pi
0.0386
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GAATGGCGAAACGAAAAGGG GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1249-1380 bp 0.833333 83.9
trnE-UUC-trnT-GGU_p2 GGCGAAACGAAAAGGGAAAG GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1245-1376 bp 0.833333 83.7
trnE-UUC-trnT-GGU_p3 CGCCTGGGAAACATAGAGTC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 1512-1549 bp 0.75 75.4
trnE-UUC-trnT-GGU_p4 GAATGGCGAAACGAAAAGGG TGGTGATTCCAGAGACGACT 50.0 50.0 58.0 58.1 1616-1652 bp 0.75 75.3
trnE-UUC-trnT-GGU_p5 GGCGAAACGAAAAGGGAAAG TGGTGATTCCAGAGACGACT 50.0 50.0 58.0 58.1 1612-1648 bp 0.75 75.1