Hotspot

trnK-UUU-rps16

LSC · 5061-5771 · 711 bp

Back to Ligusticum

Pi 0.0284
MarkerSeek score 73.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
711
variable_sites
78
pi
0.0284
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 857-1052 bp 0.916667 86.0
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 918-1113 bp 0.916667 85.8
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 917-1112 bp 0.916667 85.8
trnK-UUU-rps16_p4 CCCTTTCAGGATCAGTCGTG AAGTGTATCACGCGGGAATC 55.0 50.0 58.0 58.1 1535-1561 bp 0.75 73.9
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG AAGTGTATCACGCGGGAATC 55.0 50.0 58.0 58.1 1534-1560 bp 0.75 73.9