Hotspot

ndhB

Genome · 144302-146516 · 2215 bp

Back to Ligusticopsis

Pi 0.148

MarkerSeek score 64.30

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2215
variable_sites: 904
pi: 0.148
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	GTTGCGGAAGAACGGACTAT	AAGCCCAAAGAGGTGAATGG	50.0	50.0	58.0	58.1	2736-2874 bp	0.214286	51.9
ndhB_p2	GTTGCGGAAGAACGGACTAT	ATATAGGCCCGAGTTGGACA	50.0	50.0	58.0	57.9	2855-2993 bp	0.214286	51.5
ndhB_p3	GTTGCGGAAGAACGGACTAT	TATAGGCCCGAGTTGGACAT	50.0	50.0	58.0	57.9	2854-2992 bp	0.214286	51.5
ndhB_p4	TAGCCGGAAAGTTATGTGGC	AAGCCCAAAGAGGTGAATGG	50.0	50.0	58.0	58.1	2766 bp	0.142857	44.9
ndhB_p5	TAGCCGGAAAGTTATGTGGC	TATAGGCCCGAGTTGGACAT	50.0	50.0	58.0	57.9	2884 bp	0.142857	44.7
ndhB_p1	AAGCCCAAAGAGGTGAATGG	GTTGCGGAAGAACGGACTAT	50.0	50.0	58.1	58.0	2736-2874 bp	0.214286	51.9
ndhB_p2	ATATAGGCCCGAGTTGGACA	GTTGCGGAAGAACGGACTAT	50.0	50.0	57.9	58.0	2855-2993 bp	0.214286	51.5
ndhB_p3	TATAGGCCCGAGTTGGACAT	GTTGCGGAAGAACGGACTAT	50.0	50.0	57.9	58.0	2854-2992 bp	0.214286	51.5
ndhB_p4	AAGCCCAAAGAGGTGAATGG	TAGCCGGAAAGTTATGTGGC	50.0	50.0	58.1	58.0	2766 bp	0.142857	44.9
ndhB_p5	TATAGGCCCGAGTTGGACAT	TAGCCGGAAAGTTATGTGGC	50.0	50.0	57.9	58.0	2884 bp	0.142857	44.7