Hotspot

ycf2-ndhB

Genome · 92479-93895 · 1417 bp

Back to Ligusticopsis

Pi 0.0567

MarkerSeek score 72.20

Primer available yes

Label ycf2-ndhB

Feature Metadata

label_name: ycf2-ndhB
feature_type: igs
region: Genome
length_bp: 1417
variable_sites: 171
pi: 0.0567
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-ndhB_p1	CAGCCCTTTGTCTCTGTGTT	ATCACGGGTTCTTCTCTCCA	50.0	50.0	58.0	58.1	1729-2033 bp	0.214286	54.0
ycf2-ndhB_p2	CAGCCCTTTGTCTCTGTGTT	TCACGGGTTCTTCTCTCCAT	50.0	50.0	58.0	58.1	1728-2032 bp	0.214286	54.0
ycf2-ndhB_p3	GTTCTTGCAGAGTGGAACCA	ATCACGGGTTCTTCTCTCCA	50.0	50.0	58.0	58.1	1853-2157 bp	0.214286	53.9
ycf2-ndhB_p4	GTTCTTGCAGAGTGGAACCA	TCACGGGTTCTTCTCTCCAT	50.0	50.0	58.0	58.1	1852-2156 bp	0.214286	53.9
ycf2-ndhB_p5	GGCAAGCGGATCCTTTATGA	TCACGGGTTCTTCTCTCCAT	50.0	50.0	58.0	58.1	1905-2209 bp	0.214286	53.8