Hotspot

rps4-trnT-UGU

Genome · 46671-47037 · 367 bp

Back to Ligusticopsis

Pi 0.0103

MarkerSeek score 64.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: Genome
length_bp: 367
variable_sites: 15
pi: 0.0103
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	505-532 bp	1.0	83.0
rps4-trnT-UGU_p2	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	475-502 bp	1.0	83.0
rps4-trnT-UGU_p3	AGATCACTTCCGGCTCTAGG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	473-500 bp	1.0	82.3
rps4-trnT-UGU_p4	GTTAGTCCCGGTAAAGCTCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.7	57.8	443-470 bp	1.0	82.3
rps4-trnT-UGU_p5	TTTTTGAAACGAGGTCCCCG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.7	57.9	442-469 bp	1.0	80.1