Hotspot

clpP

LSC · 69009-71037 · 2029 bp

Back to Ligularia

Pi 0.000876

MarkerSeek score 51.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2029
variable_sites: 8
pi: 0.000876
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGACGCGAAATACAGGCT	AAGGCTTTGTTACGTTTCCA	45.0	40.0	58.0	55.8	2111-2137 bp	1.0	63.0
clpP_p2	CTCGGCAGGGTTAACTTCAA	AAGGCTTTGTTACGTTTCCA	50.0	40.0	57.8	55.8	2138-2164 bp	1.0	61.7
clpP_p3	AACTCGGCAGGGTTAACTTC	AAGGCTTTGTTACGTTTCCA	50.0	40.0	57.8	55.8	2140-2166 bp	1.0	61.7
clpP_p4	ATTGACGCGAAATACAGGCT	AAAGGCTTTGTTACGTTTCCA	45.0	38.1	58.0	56.6	2112-2138 bp	1.0	61.2
clpP_p5	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCAT	45.0	40.0	58.0	55.6	2110-2136 bp	1.0	61.1