Hotspot

atpB-rbcL

LSC · 53988-54703 · 716 bp

Back to Ligularia

Pi 0.00287

MarkerSeek score 50.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 8
pi: 0.00287
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGGTTTGTTTT	GCTTTGAATCCAACACTCGC	45.0	50.0	58.0	58.0	847-849 bp	1.0	80.8
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.0	58.0	891-893 bp	1.0	80.5
atpB-rbcL_p3	CGTGGTAACCGCAGAACTAG	GCTTTGAATCCAACACTCGC	55.0	50.0	58.4	58.0	821-823 bp	1.0	77.8
atpB-rbcL_p4	GATACGCCCCAGGTTTGTTT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.5	58.0	848-850 bp	1.0	77.1
atpB-rbcL_p5	CATTTTGCCTGGCGGAAAAG	GCTTTGAATCCAACACTCGC	50.0	50.0	58.6	58.0	899-901 bp	1.0	76.2