Hotspot

ndhC-trnV-UAC

LSC · 50239-50993 · 755 bp

Back to Ligularia

Pi 0.00324

MarkerSeek score 53.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 11
pi: 0.00324
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCTTTGCTAATCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.1	57.8	899-916 bp	1.0	72.2
ndhC-trnV-UAC_p2	TCGGAGCTAAAAATCCCGAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	56.9	57.8	888-905 bp	1.0	71.0
ndhC-trnV-UAC_p3	CCCTTTGCTAATCGGAGCTAA	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.8	57.8	899-916 bp	1.0	69.9
ndhC-trnV-UAC_p4	CCCTTTGCTAATCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.1	57.5	898-915 bp	1.0	69.5
ndhC-trnV-UAC_p5	GGCCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	57.9	50.0	58.3	57.8	901-918 bp	1.0	69.1